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Teaming Up for Clean WaterA WATER QUALITY STUDYRespect Rule: Look, Listen, Learn, and Leave Alone (until instructed).OverviewTo understand the importance of water, look beyond its liquid, invisible nature, and test its inherent properties, its essential qualities. Water quality scientists rely on five parameters to give them a snapshot of water’s nature. These parameters are temperature, dissolved oxygen, pH, turbidity, and conductivity. Measuring these parameters in the laboratory and understanding their importance to stream organisms will help students prepare for field work, and future study of stream ecology. BackgroundWater is taken for granted. For a liquid that is critical to survival, and which is taken from the tap, from bottles flavored, caffeinated, mineralized or carbonated, very little is known about the true nature of water. Is water just a clear liquid essential for survival? What is in water, and what makes it healthy for aquatic animals? Protecting the water quality of streams in California is the responsibility of the State Water Resources Control Board, or Water Board. This state agency has a program called the Clean Water Team which encourages citizens to protect their local waterways. During this study of water quality, students will be part of the Clean Water Team. The fact sheets used by students for research are developed by the Water Board, and available on their website at: This lesson focuses on five measurements of water quality often taken by aquatic biologists when assessing stream health. These parameters, temperature, dissolved oxygen, pH, turbidity, and conductivity, are critical to the survival of aquatic organisms. They are not the most important parameters for the quality of drinking water. However, they are readily measured and are a valuable prelude to more detailed laboratory experimentation in a high school, college, or commercial chemistry lab. Temperature Temperature will vary naturally primarily with the energy of sunlight, water flow, and depth of water. On dammed streams, like the Mokelumne River and Stanislaus River, temperature can be dependent on the regulation of flow from dams and the depth at which water was removed from the upstream reservoir. Water released from the bottom will be colder than water spilled from the surface. A stream’s temperature can inadvertently be changed in numerous ways. Removing riparian vegetation for flood control can reduce shade provided by overhanging limbs. Channelizing a stream for flood control can alter stream flow, reduce the depth of pools or reduce groundwater recharge. Water diversions and dams can also affect temperature. Soil erosion can increase temperatures since soil particles absorb heat. An accurate picture of stream temperature and its effect on stream health is a difficult monitoring task unless there are automated, computerized recorders that take ongoing, regular temperature measurements. However, temperature is an important measurement to monitor when sampling a creek because of its influence on oxygen solubility and its importance to aquatic organisms. Dissolved Oxygen Chemistry classes can discuss important chemical principles such as the Ideal Gas Law and Henry’s Law. Remember that the Ideal Gas Law states that the volume of a gas is inversely proportional to pressure, and directly proportional to temperature. And Henry’s Law states that the concentration of a gas dissolved in a solution is directly proportional to the partial pressure of the gas above the solution. Reducing dissolved oxygen can kill sensitive species, reduce the growth of organisms, or prevent egg hatching. D.O. may vary diurnally with temperature change, and algal photosynthesis and respiration. D.O. may be reduced by human activities or pollutants. Several factors that may reduce the D.O. are removal of riparian vegetation that shades the creek, increased nutrients and subsequent algal blooms, and increased sediment. Sediment may enter a stream if construction, logging, dirt roadways, or other soil erosion factors cause sediment loaded water to run off the watershed and into a stream. Once in the stream, sediment captures heat, temperatures can thus rise, causing oxygen levels to decline. Measuring dissolved oxygen requires a titration called the Winkler Method. A dissolved oxygen kit, including sample vials and reagents, can be purchased from chemical supply companies such as LaMotte or Hach. Kits are also available locally through the Upper Mokelumne River Watershed Council at the Central Sierra Resource Conservation and Development office in Jackson. For chemistry classes, the titration equations (below) will be of interest. More simply put, the titration uses several reactions to produce iodine in equal concentration to the original dissolved oxygen. The iodine can be observed in the reaction vessels, and is noticeably darker in samples with high dissolved oxygen. The iodine sample is then titrated with a sulfate compound, altering the iodine to form a charged iodine which is colorless. The reaction has a very clear endpoint, and is a good example of the value of titrations in chemical analyses. Here are the equations. O2 is reduced by Mn2+ at high pH. Divalent maganous hydroxide, a brown precipitate, is formed. 4e- + 4H+ + O2 > 2H2O Upon addition of iodine and H2SO4 to create an acid environment, MnO2 oxidizes I-, 4e- + 2 MnO2(s) + 8H+ > 2Mn2+ + 4H2O to form a yellow brown solution. The I2 is then titrated with thiosulfate, S2O32-, to form I- and tetrathionate, S4O62-. 2e- + I2 > 2I- The endpoint is enhanced visually by the addition of a blue starch indicator. pH Alkalinity is a slightly different measurement than pH. Alkalinity tells how well water can withstand a change in pH if an acid is added. Most natural aquatic systems have a pH range between 4 and 9. The pH of the outside environment will affect cellular reactions inside aquatic organisms. Most freshwater organisms live within the range of 6.5 to 8.5. However, some fish such as carp and catfish can tolerate higher pHs. Natural environmental factors affect the pH of water. Streams carve their way through rocks of different types, such as granite and limestone. The chemical nature of these rocks will determine the minerals that are leached from the rocks into the stream. Areas rich in carbonate minerals such as limestone will help buffer a stream’s pH. Granite soil contains few buffering agents and streams running through granite areas are more susceptible to acidic pollution such as smog. Tree leaves dropping into a stream can alter pH. Pine needles and oak leaves are acidic, while maple leaves are more basic. Waters with high algal blooms can show a diurnal change in pH. When algae grow and reproduce they use carbon dioxide. This loss of CO2 causes the pH to increase and the water to become more basic. pH will increase at the height of photosynthesis when temperatures are warmest and decline at night when algae are respiring and using CO2. These algal changes in pH are most problematic in standing waters or pools with high algal growth. Turbidity Streams erode and transport sediment downstream. Views of the Mokelumne River canyon from Highway 49 give Sierra residents an idea of how water can carve away soil and move rocks. This natural process is accelerated in stormy weather, thus local streams appear muddier after storms. Sediment can be suspended in the water during high flows and then settle out in low flows. The natural process of transporting sediment obviously varies depending on the river system. A large river, like the Sacramento River, flowing through a long, large flat valley, naturally carries more sediment than a river like the American River, where much of the upstream sediment is trapped behind Folsom Dam. The difference in these two river systems can be seen in the colors of the rivers as they merge in Sacramento at Discovery Park. While sediment transport is a natural process, one of the greatest pollutants in California is sediment. Erosion from inappropriate land practices can add too much sediment to a stream. Logging on steep slopes in the riparian corridor can cause erosion and sedimentation in streams. Overgrazing in the riparian corridor or failure of appropriate barriers on large construction sites may also cause high sediment load. Once in the stream, suspended sediment can affect stream health in several ways. First, sediment particles absorb heat and can increase temperature. Second, sediment settles onto the gravel and cobble of a stream bed. Sediment may smother fish eggs nestled in the gravel of a stream bed. A rough estimate of turbidity can be determined in the field using a transparency tube. The student fills a long cylinder with stream water and then views a dark dot at the bottom of the tube. The clarity of the dot is compared to water standards of a known turbidity. This method could be compared to a laboratory measurement if a turbidity meter is available. These meters pass a light through the water sample and measure the amount of light absorbed by the sample. Conductivity Conductivity will vary depending on the geology of the watershed. Streams following through sedimentary rocks like limestone will have higher conductivity than streams following through granite deposits. The most important anions in streams are generally bicarbonate (HCO3-), chloride (Cl-), and sulfate (SO42-). Changes in conductivity can indicate a pollution source. If a sewage pipe ruptures, raw sewage would have a much higher conductivity than the stream water. Industrial or agricultural waste (e.g. animal waste, wastewater from food processing) would also have high conductivities. Conductivity is measured using a small electrical probe. A drop in voltage between the two electrodes measures resistance, which is the reciprocal of conductivity. Conductivity is measured in “mho” because it is the inverse of “ohm,” the unit of resistance. The natural levels of conductivity in water fall in the one thousandths (mmhos) or one millionths of a mho (umhos) over a certain area (cm). Thus, the units observed in the field are in umhos/cm. Tap water ranges between 50 and 800 umhos/cm.
“If facts are the seeds that later
produce knowledge and wisdom, then the emotions and the impressions of the senses are the fertile soil in which the seeds must grow.” Rachel Carson Before-the-Field-Trip ActivitiesActivity 1: Water Quality Research
Activity 2: Water Quality Measurements
Activity 3: Water Quality Experiments
Field Trip ActivityActivity: Sampling Design
After-the-Field-Trip ActivityActivity: Water Quality Field Report
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ObjectivesStudents will:1. measure five parameters in water: temperature, dissolved oxygen, pH, turbidity, and conductivity; 2. design an experiment to evaluate these parameters in different water sources, or in different experimental conditions; 3. travel to a stream to monitor these parameters. Grade Levels712 Adult/Student RatioNormal class size LocationClassroom wet laboratory (creeks must have safe public access). Water should be flowing but low enough for students to safely sample from streamside. SkillsAnalyzing, formulating hypotheses and questions, generalizing, graphing, predicting, researching, writing a report in scientific format Key WordsAlgal, Conductivity, Dissolved oxygen, Diurnal, pH, Temperature of water, Turbidity Downloads [PDF]
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